Determination of Piperine Content in Peperomia pellucida (L.) Kunth and Peperomia obtusifolia (L.) A. Dietr

Syumillah - Saepudin (1) , Ita - Inayah (2) , Shinta Dewi Kumalasari (3) , Putri Gina Rotul Huda (4)
(1) Department of Pharmacy, Science and Math Faculty, Al Ghifari University, Bandung, Indonesia,
(2) Department of Pharmacy, Science and Math Faculty, Al Ghifari University, Bandung, Indonesia,
(3) Department of Pharmacy, Science and Math Faculty, Al Ghifari University, Bandung, Indonesia,
(4) Department of Pharmacy, Science and Math Faculty, Al Ghifari University, Bandung, Indonesia
https://doi.org/10.53675/icosei.v1i1.1719
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Issue
Vol. 1 No. 1 (2025)
Submitted
2025-10-22
Published
2025-08-14
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Abstract

Piperine is an alkaloid compound found in plants of the Piperaceae family and is known to exhibit pharmacological effects such as anti-arthritic, anti-aging, and anticancer activities. Peperomia pellucida (L.) Kunth and Peperomia obtusifolia (L.) A. Dietr are members of the Piperaceae family with potential to contain piperine. This study aimed to analyze the piperine content in the extracts and fractions of both plants using UV-Visible spectrophotometry. Extraction was performed by maceration using 70% ethanol, followed by liquid-liquid extraction with water, ethyl acetate, and n-hexane as solvents. Quantification of piperine was conducted using UV-Visible spectrophotometry with methanol as the blank, and measurement was performed at a wavelength of 343.60 nm. The results showed that the ethyl acetate fractions of Peperomia pellucida (L.) Kunth and Peperomia obtusifolia (L.) A. Dietr contained the highest piperine concentrations, at 52.981 mg PE/g and 44.413 mg PE/g, respectively

Authors

Syumillah - Saepudin
Department of Pharmacy, Science and Math Faculty, Al Ghifari University, Bandung, Indonesia
https://orcid.org/0009-0009-0632-6267 (unauthenticated)
Ita - Inayah
Department of Pharmacy, Science and Math Faculty, Al Ghifari University, Bandung, Indonesia
Shinta Dewi Kumalasari
Department of Pharmacy, Science and Math Faculty, Al Ghifari University, Bandung, Indonesia
Putri Gina Rotul Huda
Department of Pharmacy, Science and Math Faculty, Al Ghifari University, Bandung, Indonesia

How to Cite

Determination of Piperine Content in Peperomia pellucida (L.) Kunth and Peperomia obtusifolia (L.) A. Dietr. (2025). Proceeding International Conference On Sustainable Environment And Innovation (ICOSEI), 1(1). https://doi.org/10.53675/icosei.v1i1.1719

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