ANALISIS KADAR TOTAL FITOSTEROL PADA EKSTRAK DAUN SELADA (Lactuca sativa L.)DENGAN METODE KOLORIMETRI

Irma Erika Herawati, Nyi Mekar Saptarini

Abstract


Daun selada (Lactuca sativa L.) mengandung fitosterol, yang secara empirik bisa digunakan sebagai anti kolesterol. Fitosterol dapat digunakan dalam mengurangi absorpsi kolesterol di dalam saluran pencernaan, hal ini karena fitosterol memiliki struktur yang mirip dengan kolesterol. Penelitian ini bertujuan untuk mengetahui kadar total fitosterol pada daun selada. Ekstraksi yang dilakukan adalah metode maserasi dengan menggunakan pelarut n-heksan:etanol. Penentuan kadar total kolesterol menggunakan metode kolorimetri, yaitu pembentukan warna hijau dari senyawa sterol dengan pereaksi Liebermann-Burchard. Dari hasil penelitian didapatkan bahwa kadar fitosterol total daun selada sebesar 17,42 mg±0,7841/100g simplisia kering.


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