VALIDASI PARSIAL UJI PENETAPAN KADAR VIRULENSI (Vi) PADA PRODUK RUAHAN POLISAKARIDA VIRULENSI (Vi), PRODUK RUAHAN KONJUGAT VIRULENSI- DIFTERIA (ViDT), DAN PRODUK AKHIR VAKSIN TIFOID KONJUGAT DENGAN METODE HPAEC- PAD

Ginayanti Hadisoebroto (1) , Siti Azizah (2) , Atin Supriyatin (3)
(1) Prodi Farmasi, Universitas Al-Ghifari,
(2) PT. Biofarma Bandung,
(3) Prodi Farmasi, Universitas Al-Ghifari
https://doi.org/10.53675/jsfar.v2i1.27
crossmark

##plugins.themes.bootstrap3.article.sidebar##

Terbitan
Vol 9 No 1 (2021): SABDARIFFARMA
Telah diserahkan
2020-07-11
Diterbitkan
2020-06-11
PDF (Inggris)

Abstrak

Vaksin konjugat dibuat dengan menggunakan bagian kapsul bakteri. Kapsul tersebut mengandung antigen polisakarida Vi, di mana Vi merupakan faktor untuk menentukan tingkat patogen di dalam bakteri dan antigen merupakan molekul struktur turunan dari bakteri yang dapat memicu respon imun protektif ketika dikombinasikan dengan molekul struktur yang lebih besar seperti carrier protein. Antigen polisakarida Vi berhubungan dengan imunogenisitas yang merupakan kemampuan suatu imunogen untuk menginduksi respon imun di dalam tubuh. Maka dari itu kadar Vi diuji untuk melihat dan menilai seberapa efektif dan protektif vaksin typhoid Vi jenis konjugasi ini. Metode HPAEC-PAD adalah kromatografi pertukaran anion berkinerja tinggi untuk kuantifikasi kandungan sakarida bebas dan total dari vaksin konjugasi tifoid (konsentrat bulk atau fill akhir) sakarida yang dipisahkan dideteksi dengan Pulsed Amperometric Dection (PAD). Metode tersebut perlu dilakukan validasi untuk menghasilkan data yang diyakini konsisten sesuai dengan spesifikasi yang telah ditetapkan. Tujuan penelitian ini adalah membuktikan bahwa metode HPAEC-PAD yang digunakan akan memberikan ketelitian, ketepatan serta ketegaran (Robustness) yang memenuhi persyaratan.

Penulis

Ginayanti Hadisoebroto
Prodi Farmasi, Universitas Al-Ghifari
Siti Azizah
PT. Biofarma Bandung
Atin Supriyatin
Prodi Farmasi, Universitas Al-Ghifari

Cara Mengutip

VALIDASI PARSIAL UJI PENETAPAN KADAR VIRULENSI (Vi) PADA PRODUK RUAHAN POLISAKARIDA VIRULENSI (Vi), PRODUK RUAHAN KONJUGAT VIRULENSI- DIFTERIA (ViDT), DAN PRODUK AKHIR VAKSIN TIFOID KONJUGAT DENGAN METODE HPAEC- PAD. (2020). Jurnal Sabdariffarma: Jurnal Ilmiah Farmasi, 9(1), 19-24. https://doi.org/10.53675/jsfar.v2i1.27

Please find the rights and licenses in Jurnal Sabdariffarma. By submitting the article/manuscript of the article, the author(s) agree with this policy. No specific document sign-off is required.

  1. License

The non-commercial use of the article will be governed by the Creative Commons Attribution license as currently displayed on Creative Commons Attribution-Share Alike 4.0 International License. 

  1. Author(s)' Warranties

The author warrants that the article is original, written by stated author(s), has not been published before, contains no unlawful statements, does not infringe the rights of others, is subject to copyright that is vested exclusively in the author and free of any third party rights, and that any necessary written permissions to quote from other sources have been obtained by the author(s).

  1. User Rights

Sabdariffarma's spirit is to disseminate articles published are as free as possible. Under the Creative Commons license, Sabdariffarma permits users to copy, distribute, display, and perform the work for non-commercial purposes only. Users will also need to attribute authors and Sabdariffarma on distributing works in the journal and other media of publications.

  1. Rights of Authors

Authors retain all their rights to the published works, such as (but not limited to) the following rights;

  • Copyright and other proprietary rights relating to the article, such as patent rights,
  • The right to use the substance of the article in own future works, including lectures and books,
  • The right to reproduce the article for own purposes,
  • The right to self-archive the article,
  • The right to enter into separate, additional contractual arrangements for the non-exclusive distribution of the article's published version (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal (Jurnal Sabdariffarma).
  1. Co-Authorship

If the article was jointly prepared by more than one author, any authors submitting the manuscript warrants that he/she has been authorized by all co-authors to be agreed on this copyright and license notice (agreement) on their behalf, and agrees to inform his/her co-authors of the terms of this policy. Sabdariffarma will not be held liable for anything that may arise due to the author(s) internal dispute. Sabdariffarma will only communicate with the corresponding author.

  1. Royalties

Being an open accessed journal and disseminating articles for free under the Creative Commons license term mentioned, author(s) aware that Sabdariffarma entitles the author(s) to no royalties or other fees. 

  1. Miscellaneous

Sabdariffarma will publish the article (or have it published) in the journal if the article’s editorial process is successfully completed. Sabdariffarma's editors may modify the article to a style of punctuation, spelling, capitalization, referencing and usage that deems appropriate. The author acknowledges that the article may be published so that it will be publicly accessible and such access will be free of charge for the readers as mentioned in point 3.

Privacy Statement

The names and email addresses entered in this journal site will be used exclusively for the stated purposes of this journal and will not be made available for any other purpose or to any other party.

##plugins.themes.bootstrap3.article.details##

Referensi

Alba S, Bakker MI, Hatta M, Scheelbeek PFD, et al.. 2016. Risk Factors of Typhoid Infection in the Indonesian Archipelago. PLoS ONE 11(6): e0155286. doi:10.1371/journal.pone.0155286.

Anonim. (2003). The Diagnosis, Treatment and Prevention of Thypoid Fever. Retrieved Februari 18, 2018, from World Health Organization- Vaccines Document.

Buckle GC, Walker CLF, dan Black RE. 2012. Typhoid fever and paratyphoid fever: Systematic review to estimate global morbidity and mortality for 2010. J Glob Health. 2(1):10401.

Erman Tritama. 2015. Development of Typhoid Conjugate Vaccine in Biofarma. In 9th International Conference on Typhoid and Invasive NTS Disease.

McGregor AC, Waddington CS, Pollard AJ. 2013. Prospects for prevention of Salmonella infection in children through vaccination. Curr. Opin. Infect. Dis. 26: 254–262.

Micoli, F., Rondini, S., Pisoni, L., Berti, F., Proietti, D., Costantino, P., et al. (2011). CRM 197 As A New Conjugate Vaccine Against Salmonella Thypi. Vaccine, 712-720.

Rohrer, J. 2004. Analysis of Carbohydrates by High Performance Anion- Exchange Chromatography with Pulsed Amperometric Detection.

Artikel paling banyak dibaca berdasarkan penulis yang sama

1 2 > >>